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©2014 Baishideng Publishing Group Co.
World J Stem Cells. Jan 26, 2014; 6(1): 11-23
Published online Jan 26, 2014. doi: 10.4252/wjsc.v6.i1.11
Published online Jan 26, 2014. doi: 10.4252/wjsc.v6.i1.11
Table 4 Three-dimensional natural and synthetic extracellular matrices scaffolds for neural differentiation of pluripotent stem cells
Scaffolds | Cell source | Neural differentiation | Ref. |
Poly(lactic-co-glycolic acid) and poly(L- lactic acid) scaffolds | hESC | Enhanced numbers of neural structures and staining of nestin and β-tubulin III were observed | Levenberg et al[74], 2005 |
Synthetic hydrogel matrix PuraMatrix | hESC-derived neuronal cells | HESC-derived neurons, astrocytes, and oligodendrocytes grew, matured and migrated in hydrogel; neuronal cells had electrically active connections. | Ylä-Outinen et al[76], 2012 |
Poly(epsilon-caprolactone)-poly(beta-hydroxybutyrate) scaffolds | Mouse iPSCs | Improved iPSCs to differentiate into neurons and inhibited other differentiations. | Kuo et al[79], 2012 |
Polycaprolactone nanofiber matrices | HESC-derived neural precursors | Aligned fibrous matrices showed higher rate of neuronal differentiation compared to random micro- and nano- fibers (62%-86% vs 27%-32%). | Mahairaki et al[77], 2011 |
Polyurethane nanofibrous scaffolds | hESC line SA002 | Neuronal differentiation was preferred over astrocyte differentiation. | Carlberg et al[80], 2009 |
Tissue-engineered fibrin scaffolds | Mouse ESC-derived NPCs | Enhanced NPC survival and directed differentiation into neurons. | Johnson et al[82], 2010 |
PET microfibrous scaffolds | Mouse ESC D3 line | Enhanced neuronal differentiation indicated by nestin, Nurr1, and tyrosine hydroxylase compared to 2-D culture. | Liu et al[84,85], 2013, 2013 |
Multiwalled carbon nanotube modified PET microfibrous scaffolds | Mouse ESC D3 line | Enhanced neuronal differentiation compared to unmodified scaffolds | Zang et al[83], 2013 |
Chitin-alginate 3-D microfibrous scaffolds | hESC line: HUES 7 hiPSC lines: PD-iPS5 and hFib2-iPS4 | Efficient neuronal differentiation: > 95% nestin+; able to mature into neurons (> 90% β-tubulin III+) | Lu et al[75], 2012 |
3-D ECM scaffolds derived from ESC aggregates | Mouse ESC D3 line | ECM scaffolds derived from RA-treated EBs enhanced nestin and β-tubulin III expressions | Sart et al[60], 2013 |
Inverted colloidal crystal (ICC) scaffolds containing alginate, poly(gamma- glutamic acid), and surface peptide; or chitin-chitosan-gelatin ICC scaffolds | Mouse iPSCs | Accelerated neuronal differentiation (β-tubulin III expression) of iPSCs. | Kuo et al[86,87], 2013, 2013 |
- Citation: Li Y, Liu M, Yan Y, Yang ST. Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix. World J Stem Cells 2014; 6(1): 11-23
- URL: https://www.wjgnet.com/1948-0210/full/v6/i1/11.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v6.i1.11