Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene

Figure 10 In vivo animal study of hyperglycemia. Treatment by transplantation of human adipose tissue derived stem cells^PDX1+ and human adipose tissue derived stem cells (4 × 10⁶ cells/rat). Before alloxan injection, the blood glucose level was 180 ± 20 and it reached 500 ± 20 mg/mL two weeks after injection. Six alloxan induced diabetic rats were transplanted with human adipose tissue derived stem cells (hADSCs)^PDX1+ which resulted in decreasing the blood glucose level to 200 ± 20 mg/mL after 3 d. The blood glucose levels of 6 rats transplanted with hADSCs were decreased transiently to 300 ± 20 mg/mL after 10 d and increased again. Control group which consisted of 6 diabetic rats untransplanted were hyperglycemic and died within 6 mo.