Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene

Figure 8 The immunocytochemistry experiments using PDX1 and insulin antibodies 7 d after transduction of human adipose tissue derived stem cells with non-integrated lentiviruses harboring PDX1 and selection of transduced cells with puromycin. FITC conjugated PDX1 antibody (green color) and FITC conjugated insulin antibody (green color) were used for immune staining of PDX1 and insulin proteins and DAPI (blue color) was used for nuclear staining. A: Untransduced human adipose tissue derived stem cells (hADSCs) represented no green signals; B: hADSCs^PDX1+ represented green signals related to insulin expression in the cytoplasm; C: hADSCs^PDX1+ represented green signals related to PDX1 expression in the nucleus.