Human adipose tissue contains erythroid progenitors expressing fetal hemoglobin

Figure 3 Representative flow cytometric dot plots of hemoglobin expression in erythroid cells. Burst-forming units-erythroid (BFU-E)-derived colonies were analyzed for HbF and HbA expression by flow cytometry after different lengths of time in culture. Erythroid cells generated from cord blood (CB) CD34⁺ cells (A), from peripheral blood (PB) CD34⁺ cells (B), and from stromal vascular fraction (SVF) cells (C). Representative fluorescence-activated cell sorting plots showing hemoglobin expression in circulating erythrocytes from CB and PB (D). Representative phase-contrast photomicrographs showing the morphological aspect of the BFU-Es generated from CD45^- (left) and CD45⁺(right) cells isolated from SVF (E). HbF: Fetal hemoglobin; HbA: Adult hemoglobin; CB: Cord blood; PB: Peripheral blood.