Mobilization of CD34+CD38- hematopoietic stem cells after priming in acute myeloid leukemia

Figure 1 Multicolor flow cytometry (patient # 3): (left) Strategy used for gating the blast cell population and CD34⁺CD38 cell subpopulations (red: immature CD45^low/SSC blast cells, blue: CD34⁺ cells, green: lymphocytes, blue cyan: monocytes, violet: granulocytes, black: CD45⁺ cells (erythroblasts); CD34⁺ cells were separated into different stem cell fractions based on their CD38 antigen expression: A first cell population expressing a great amount of the CD34 antigen and lacked of CD38 (CD34⁺CD38^-), a second cell population characterized by a great amount of the CD34 antigen and by a low density of CD38 antigen (CD34⁺CD38^low/), and a third cell population characterized by a large density of CD38 antigen and of CD34 antigen (CD34⁺CD38⁺); (right) The two density plots indicate CD45low/SSC blast cells (in red) and CD34⁺CD38 subpopulations (CD34⁺CD38^- in orange, CD34⁺CD38^low in green and CD34⁺CD38⁺ in blue).