Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells

Figure 2 Effect of nutritional supplements added to SB medium on HES-3 differentiation to cardiomyocytes. Differentiation efficiency is based on two FACS markers: myosin heavy chain (MHC) and sarcomeric α-actinin (SA). Cell and cardiomyocyte (CM) yields were measured at day 16 of culture. Results were normalized against the yields obtained in SB medium (control). (n = 3, ^aP < 0.05, ^bP < 0.01 vs cell yield). BSA: Bovine serum albumin; HySoy: Soy-hydrolysate.