Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells

doi:10.4252/wjsc.v5.i3.86

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jul 26, 2013; 5(3): 86-97
Published online Jul 26, 2013. doi: 10.4252/wjsc.v5.i3.86

Table 2 Quantitative real-time reverse transcription-polymerase chain reaction primers

Primers for qPCR	Forward (5’→3’)	Reverse (5’→3’)
Nanog	GAAAAACAACTGGCCGAAGAAT	GGTGCTGAGGCCTTCTGC
4-Oct	AACGACCATCTGCCGCTTT	GGCCGCAGCTTACACATGTT
T-bra	AATTTGGTCCAGCCTTGGAAT	CGTTGCTCACAGACCACAG
Mesp 1	GACGTGCTGGCTCTGTTG	TGTCACTTGGGCTCCTCAG
Nkx 2.5	CAAGTGTGCGTCTGCCTTT	TTGTCCGCCTCTGTCTTCTC
MHC	ATTGCTGAAACCGAGAATGG	CGCTCCTTGAGGTTGAAAAG
GAPDH	GTCGGAGTCAACGGATTTGG	AAAAGCAGCCCTGGTGACC

qPCR: Quantitative polymerase chain reaction; MHC: Myosin heavy chain; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

Citation: Ting S, Lecina M, Chan YC, Tse HF, Reuveny S, Oh SK. Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells. World J Stem Cells 2013; 5(3): 86-97
URL: https://www.wjgnet.com/1948-0210/full/v5/i3/86.htm
DOI: https://dx.doi.org/10.4252/wjsc.v5.i3.86