Reprogramming somatic cells by fusion with embryonic stem cells does not cause silencing of the Dlk1-Dio3 region in mice

Figure 3 DNA methylation of intergenic differentially methylated region in embryonic stem cells (tauGFP), MEFs, tef4, taf2, tef9, and taf5. (A) Structure of the Dlk1-Dio3 locus with the position of the intergenic differentially methylated region (IG DMR) analyzed by bisulfite sequencing (CpGs indicated by circles) and pyrosequencing (indicated by black bar). Numbers indicate position relative to the Gtl2 transcription start site; (B) Percentage of DNA methylation of CpG-sites within IG DMR in tef4, taf2, tef9, and taf5 hybrid clones, as well as parental tauGFP embryonic stem (ES) cells (ESC) and fibroblasts (MEFs), based on the data of pyrosequencung.