Cell-free derivatives from mesenchymal stem cells are effective in wound therapy

Figure 5 SDF-1 cytokine measurement in human mesenchymal stem cells co-cultured with keratinocytes. A: SDF-1 cytokine secretion was measured in conditioned medium (CM) obtained from human mesenchymal stem cells (hMSCs) co-cultured with keratinocytes (MSC + KRT CM) compared with MSC (MSC CM) (P≤ 0.0004; n = 3) and keratinocytes (KRT CM) alone for 48 h using Multiplex assay. Increased CXCL5 (ENA-78) protein expression; B: Enzyme-linked immunosorbent assay for CXCL5 protein was performed on cell-free conditioned medium (CM) obtained from cocultured hMSCs + Keratinocytes (KRT + MSC CM) and compared with hMSC (MSC CM) alone (P≤ 0.0002; n = 3) and Keratinocytes (KRT CM) alone (P < 0.0001; n = 3) respectively. Increased expression of SDF-1 and CXCL5 was also observed in wounded skin injected with hMSC and hMSC lysate; C: SDF-1 and CXCL5 expression levels were increased in sections from hMSC and hMSC lysate injected skin (wounded) as compared with naturally healing (wounded) skin and normal skin, GAPDH was utilized as an internal control. ^aIndicates statistically significant difference.