Dynamic culture improves MSC adhesion on freeze-dried bone as a scaffold for bone engineering

Figure 5 Cell viability after dynamic or static co-culture, measured by the exclusion method with Trypan Blue. The cell viability ratio was assessed on days 0, 3 and 6 after cultures. On day 0, the cell proliferation potential in the dynamic system was higher than in the static system. Over days 0, 3 and 6 after dynamic system, there was a decrease in cell viability on bone matrix in prolonged non-dynamic culture. In the static system, the cell proliferation on day 6 was significantly lower than on day 3 and 0. Three independent experiments were performed for each co-culture system. ^aP < 0.05.