Umbilical cord blood mesenchymal stem cells protect amyloid-β42 neurotoxicity via paracrine

Figure 3 Knock-down of decorin and progranulin by siRNAs reduces the anti-apoptotic effect on human umbilical cord blood-derived mesenchymal stem cells in a Transwell chamber. A, B: Each siRNA was pretreated in human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) for 6 h. These cells were co-cultured with rat primary neuronal cells for 24 h. After co-culturing, the medium was analyzed by enzyme-linked immunosorbant assay for progranulin (A) (^aP < 0.05, n = 3 per group) and decorin (B) (^aP < 0.05, n = 3 per group); C: Rat primary neurons in each condition were stained by anti-microtubule associated protein 2 (MAP2) antibody. Red color indicates MAP2-positive neurons and nuclei were visualized by DAPI (blue); D: The percentage of MAP2-postive neuron in each conditions were analyzed (^aP < 0.05, n = 3 per group). CONT: Control; PGRN: Progranulin; DCN: Decorin.