Umbilical cord blood mesenchymal stem cells protect amyloid-β42 neurotoxicity via paracrine

Figure 1 Decorin and progranulin are highly secreted from human umbilical cord blood-derived mesenchymal stem cells. A: Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) were co-cultured with amyloid-β42-exposed rat primary neuronal cells for 24 h in a Transwell chamber. Then, rat primary neuronal cells were stained by Live/Dead staining. Green color indicates surviving neuronal cells. B: Percentage of dead cells was calculated (P < 0.05, n = 4 per group); C: To identify paracrine factors, co-cultured media was analyzed by antibody-based array (RayBio). Spot intensity of progranulin and decorin in co-cultured media was much higher compared to rat primary neuronal cells in the absence of hUCB-MSC (P < 0.05, n = 3 per group); D: Each medium used in the Transwell was analyzed by enzyme-linked immunosorbant assay for progranulin and decorin (^aP < 0.05, ^cP < 0.05, n = 3 per group).