Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method

Figure 7 Immunosuppressive effect of mouse clonal mesenchymal stem cell lines or nonclonal mesenchymal stem cells on T cells. Splenocytes (2 × 10⁵) from BALB/c were stimulated with soluble anti-CD3 (1 μg/mL) and anti-CD28 (1 μg/mL) in the presence of 1 × 10⁴ different mesenchymal stem cells. A: After 48 h, T-cell receptor-activated T-cell clusters were observed by microscopy; B: Interferon (IFN)-γ production was determined by ELISA; C: T-cell proliferation was determined by [³H]-thymidine incorporation after 3 d culture. GCM: Gradient centrifugation method.