Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method

Figure 5 Osteogenic differentiation potentials of the established mouse clonal mesenchymal stem cell lines and nonclonal mesenchymal stem cells. A: Alizarin Red S staining showed matrix mineralization in the osteogenically differentiated mouse clonal mesenchymal stem cell lines 21 d after osteogenic induction: a and b: BALB/c gradient centrifugation method (GCM); c and d: BALB/c D1-#2; e and f: BALB/c D2-#1; g and h: C3H GCM; i and j: C3H D2-#1; k and l: C3H D3-#2; m and n: C57BL/6 GCM; o and p: C57BL/6 D3-#1; q and r: C57BL/6 D3-#2; B: Reverse transcriptase-polymerase chain reaction analysis of osteogenic markers, alkaline phosphatase (ALP), Runx-2, Col1A1, and Distal-less homeobox 5 at days 0 and 7 after osteogenic induction. Glyceraldehyde phosphate dehydrogenase was used as an internal control.