Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method

Figure 4 Adipogenic differentiation potential of the established mouse clonal mesenchymal stem cell lines and nonclonal mesenchymal stem cells. A: Oil-red-O staining showed that adipogenically differentiated mouse clonal mesenchymal stem cell lines were positive, tested 4 d after adipogenic induction: a and b: BALB/c gradient centrifugation method (GCM); c and d: BALB/c D1-#2; e and f: BALB/c D2-#1; g and h: C3H GCM; i and j: C3H D2-#1; k and l: C3H D3-#2; m and n: C57BL/6 GCM; o and p: C57BL/6 D3-#1; q and r: C57BL/6 D3-#2; B: Reverse transcriptase-polymerase chain reaction analysis of the adipogenic markers peroxisome proliferator-activated receptor γ2 (PPARγ2), fatty acid-binding protein 4, lipoprotein lipase (LPL), and Col1A1 at days 0 and 4 after adipogenic induction. glyceraldehyde phosphate dehydrogenase was used as an internal control.