Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method

doi:10.4252/wjsc.v3.i8.70

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Aug 26, 2011 (publication date) through Jan 6, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Aug 26, 2011; 3(8): 70-82
Published online Aug 26, 2011. doi: 10.4252/wjsc.v3.i8.70

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Figure 3 Cell growth of the established mouse clonal mesenchymal stem cell lines and nonclonal mesenchymal stem cells. Cells were plated at 1 × 10³, 5 × 10³, or 1 × 10⁴ cells in a 60-mm dish and incubated in Dulbecco’s modified Eagle’s Medium-low glucose. Cells were trypsinized by incubation with trypsin/ethylene diamine tetraacetic acid and counted twice a week with a haemocytometer for 15 d. GCM: Gradient centrifugation method.

Citation: Jeon MS, Yi TG, Lim HJ, Moon SH, Lee MH, Kang JS, Kim CS, Lee DH, Song SU. Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method. World J Stem Cells 2011; 3(8): 70-82
URL: https://www.wjgnet.com/1948-0210/full/v3/i8/70.htm
DOI: https://dx.doi.org/10.4252/wjsc.v3.i8.70