Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method

Figure 2 Pictures of the individual colonies and cell morphology of the established mouse clonal mesenchymal stem cell lines. A: Representative pictures of single colonies obtained from different strains; B: Pictures of mouse clonal mesenchymal stem cells (mcMSCs) established from single colonies at about 70%-80% confluence. Nonclonal MSCs isolated by the conventional method, labeled as the gradient centrifugation method, from the three strains are also shown. Every mcMSCs in the right panel have a fibroblast-like morphology. The cells were fixed with 4% paraformaldehyde for 10 min and stained with 0.1% crystal violet for 5 min. Magnification: 100 ×.