Short and long term fate of human AMSC subcutaneously injected in mice

Figure 5 Paraffin-embedded tissue sections from NOD/SCID mice transplanted subcutaneously with human adipose-derived mesenchymal stem cells after 17 mo. Sections were immunostained with monoclonal anti-human mitochondria antibody (A-D) or anti-human ribonucleoprotein antibody (E, F) and then were counterstained with hematoxylin. A: Saline injected SCID showing no staining for anti-human mitochondria in spleen. Negative control; B: SCID subcutaneous adipose tissue section at the site of injection at 17 mo showing staining for anti human mitochondria in the adipocytes in brown (arrows); C: Liver of a human adipose-derived mesenchymal stem cell (AMSC) SCID injected mouse at 17 mo showing no human AMSC (hAMSC); D: Lung of a human AMSC SCID injected mouse at 17 mo showing no hAMSCs; E: Spleen of a human AMSC SCID injected mouse at 17 mo showing no hAMSCs; F: SCID subcutaneous dermal tissue section at the site of injection at 17 mo showing staining for anti human ribonucleoprotein in the fibroblasts in brown (arrows).