Short and long term fate of human AMSC subcutaneously injected in mice

Figure 1 In vitro characterization of human adipose-derived mesenchymal stem cell. A: Phase contrast of a primary subconfluent culture showing typical adherent fibroblast-like morphology; B: Anti-CD90 immunofluorescence (in red) of a human adipose-derived mesenchymal stem cell (hAMSC) culture; C: Assessment of in vitro differentiation potential of hAMSC by osteogenic differentiation, demonstrated by alkaline phosphatase activity in red; D: Assessment of in vitro differentiation potential of hAMSC by myogenic differentiation, demonstrated by anti-sarcomeric-α-actin in brown (arrow); E: Assessment of in vitro differentiation potential of hAMSC by adipogenic differentiation, demonstrated by Oil red. Arrow: fat drops in a labeled differentiated cell. Arrowhead: Nucleus of an undifferentiated cell; F: Assessment of in vitro differentiation potential of hAMSC by chondrogenic differentiation, demonstrated by Alcian blue.