Preclinical transplantation and safety of HS/PCs expanded from human umbilical cord blood

Figure 3 Engraftment of cells from hematopoietic/progenitor stem cell product in NOD/SCID mice. A: The recovery of WBC population in irradiated NOD/SCID mice transplanted with the expanded hematopoietic/progenitor stem cells (HS/PCs) and the combination of expanded HS/PCs and BM-mesenchymal stem cells (MSCs). Group A: WBC of mice transplanted with expanded HS/PCs, Group B: WBC of mice transplanted with the combination of expanded HS/PCs and BM-MSCs, Group C: WBC of mice in the control group. BL: Baseline of WBCs before irradiation; B: RT-PCR detection of human-specific Alu sequence fragment in NOD/SCID mice bone marrow. The GAPDH mRNA was used as a housekeeper gene to normalize the threshold cycle measurements for the Alu product. The result was showed as the concentration ratio of PCR product to that of GAPDH mRNA. ^aP < 0.05; C: The percentage of human CD45⁺ cells in the bone marrow of mice detected by the flow cytometry. White diamond indicates the percentage of human CD45+ cells in the bone marrow of mice transplanted with the combination of HS/PC product and BM-MSCs. Black diamond indicates the percentage of human CD45⁺ cells in the bone marrow of mice transplanted only with HS/PC product.