Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

Figure 4 Comparison of agonist-induced changes of [Ca²⁺]_i in high- and low-Ca solutions. Cell fluorescence was monitored before and after addition of agents in high-Ca (1.8 mmol/L) and low-Ca (0.6 mmol/L) Hank’s medium. Panels summarize effects of (A) 200 mmol/L ATP (B), 20 mmol/L Bradykinin (C), 100 mmol/L Histamine (D) 20 mmol/L Thapsigargin. Asterisks mark significant (P < 0.05) differences.