Spontaneous immortalization of human dermal microvascular endothelial cells

Figure 3 iHDME1 cells retain endothelial properties in vitro. A: Migration of Human dermal microvascular endothelial cells (HDMECs) and iHDME1 cells towards medium with or without VEGF (25 ng/mL). Migrating cells were counted 4 h after cell plating in eight randomly selected high power fields. ^aP < 0.05 using Student’s t test, comparing to unstimulated cells; B: iHDME1 cells in 3-D Matrigel cultures. Vascular structure formation was followed over time and representative images taken 28 h after plating are shown; C: Cross point of vascular structures from HDMECs and iHDME1 cells were counted under microscope and graphed 28 h after plating. The data were collected from three independent experiments.