Human umbilical cord mesenchymal stem cell-derived exosomal miR-199a-3p inhibits the MAPK4/NF-κB signaling pathway to relieve osteoarthritis

Figure 5 Overexpression of mitogen-activated protein kinase 4 attenuates the effects of miR-199a-3p on inflammation and apoptosis in chondrocytes. A: Mitogen-activated protein kinase 4 expression was detected by western blot; B: Interleukin-6, interleukin-8, and tumor necrosis factor-α expression in CHON-001 cells was detected via enzyme-linked immunosorbent assay; C: A CCK-8 assay was used to measure the viability of CHON-001 cells; D: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling staining was used to measure the degree of CHON-001 cell apoptosis (scale bar = 100 μm); E: Western blot analysis was used to detect Bcl-2 and Bax apoptosis-related proteins in CHON-001 cells. ^bP < 0.01, ^cP < 0.001. MAPK4: Mitogen-activated protein kinase 4; IL: Interleukin; OE-MAPK4: Overexpression mitogen-activated protein kinase 4; DAPI: 4’,6-diamidino-2-phenylindole; OD: Optical density; TUNEL: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling.