EZH2, via an association with KDM2B, modulates osteogenic differentiation of root apical papillary stem cells

Figure 5 Enhancer of zeste homolog 2 bioactive peptides effectively block lysine demethylase 2B binding to enhancer of zeste homolog 2 and promote human apical papillary stem cell osteogenic differentiation. A and B: Peptide site immunohybridization results confirmed the presence of a direct binding site between enhancer of zeste homolog 2 (EZH2) and lysine demethylase 2B; C: Gray value analysis identified 16 positive loci, with gray values ranging between 80%-100%; D: Schematic illustration of different functional domains of EZH2 protein; E: Co-immunoprecipitation assays indicated that peptides PP7 and PP8 effectively inhibited interaction between EZH2 and lysine demethylase 2B; F: Alkaline phosphatase staining demonstrated that EZH2 peptides significantly promoted osteogenic differentiation of apical papillary stem cells. Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using Student’s t-test. ^aP ≤ 0.05. EZH2: Enhancer of zeste homolog 2; KDM2B: Lysine demethylase 2B; ALP: Alkaline phosphatase.