EZH2, via an association with KDM2B, modulates osteogenic differentiation of root apical papillary stem cells

Figure 4 Reduction of enhancer of zeste homolog 2 binding to lysine demethylase 2B promotes osteogenic differentiation of human apical papillary stem cells. A: The protein-protein interaction network analysis revealed potential direct or indirect interactions between enhancer of zeste homolog 2 (EZH2) and lysine demethylase 2B (KDM2B); B: Quantitative polymerase chain reaction confirmed that EZH2 expression progressively increased, while KDM2B expression gradually decreased during the induction of osteogenic differentiation in human apical papillary stem cells; C and D: Molecular docking simulations predicted the binding modes and affinities between EZH2 and KDM2B proteins. C illustrates the two-dimensional interactions, while D presents the three-dimensional binding conformations. Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using Student’s t-test. ^aP ≤ 0.05, ^bP ≤ 0.01, ^cP ≤ 0.001. EZH2: Enhancer of zeste homolog 2; KDM2B: Lysine demethylase 2B.