EZH2, via an association with KDM2B, modulates osteogenic differentiation of root apical papillary stem cells

Figure 3 Enhancer of zeste homolog 2 inhibits expression of osteogenic differentiation-related genes independently of histone methyltransferase effect. A: Heatmap illustrating differential gene expression in human apical papillary stem cells (SCAPs) following enhancer of zeste homolog 2 (EZH2) knockdown; B: Quantitative polymerase chain reaction analysis validated the expression changes of five randomly selected genes, consistent with the findings of the mRNA transcriptome microarray analysis; C: Histogram presenting significant results of Gene Ontology analysis for differentially expressed genes in SCAPs with EZH2 knockdown compared to the control group; D: Scatter plot depicting Kyoto Encyclopedia of Genes and Genomes pathway enrichment statistics for differentially expressed genes in SCAPs with EZH2 knockdown compared to the control group; E: Venn diagram showing the intersection between genes upregulated in human apical papillary stem cells following EZH2 knockdown and those associated with reduced trimethylation levels at the H3K27 locus; F: Histogram displaying significant Gene Ontology analysis results for differentially expressed genes in SCAPs with EZH2 knockdown relative to the control group. Data are presented as the mean ± SD (n = 3). Statistical analysis was performed using Student’s t-test. ^bP ≤ 0.01, ^cP ≤ 0.001. PSAT1: Phosphoserine aminotransferase 1; STC2: Stanniocalcin 2; PCK2: Phosphoenolpyruvate carboxykinase 2; TRIB3: Tribbles pseudokinase 3; FGF2: Fibroblast growth factor 2; EZH2: Enhancer of zeste homolog 2.