L-arginine from elder human mesenchymal stem cells induces angiogenesis and enhances therapeutic effects on ischemic heart diseases

Figure 4 Verification of arginine succinate lyase overexpression and knockdown in bone marrow-derived mesenchymal stem cells. A: Bone marrow-derived mesenchymal stem cells after plasmid transfection and green fluorescent protein were observed under normal and fluorescence microscopes. Scale bars = 200 μm; B: Arginine succinate lyase (ASL) protein levels were significantly upregulated or significantly downregulated relative to the negative control group using ASL-overexpressing or ASL-short hairpin RNA plasmids; C: Relative quantitative comparison of western blot analyses (n = 3/group). ^aP < 0.05,^cP < 0.001. ASL: Arginine succinate lyase; DIC: Plasmid transfection; GFP: Green fluorescent protein; shRNA: Short hairpin RNA; BMSCs: Bone marrow-derived mesenchymal stem cells.