RNA interference-mediated osteoprotegerin silencing increases the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin ratio and promotes osteoclastogenesis

Figure 1 Cultured bone marrow-derived mesenchymal stem cells micrographs and flow cytometry analysis. A: Microscopic image of bone marrow-derived mesenchymal stem cells (BMSCs) after Giemsa staining (scale bar = 200 μm); B: Microscopic image of BMSCs stained with Oil Red O on day 21 (scale bar = 200 μm); C: Microscopic image of BMSCs stained with Alizarin Red on day 21 (scale bar = 200 μm); D and E: Flow cytometry analysis of CD73 (D) and CD90 (E) expression in cultured BMSCs; F and G: Flow cytometry analysis of CD11B (F) and CD45 (G) expression in cultured BMSCs. The results show that over 95% of cultured BMSCs express the mesenchymal stem cell markers CD73 and CD90, while less than 2% of BMSCs express the hematopoietic stem cell markers CD11B and CD45.