Exosomal miR-137-3p targets UBE3C to activate STAT3, promoting migration and differentiation into endometrial epithelial cell of human umbilical cord mesenchymal stem cells under hypoxia

Figure 4 miR-137-3p bolstered the migratory and differentiation propensities of human umbilical cord mesenchymal stem cell into endometrial epithelial cell, an effect mitigated by signal transducer and activator of transcription 3 inhibition. Human umbilical cord mesenchymal stem cell, transfected with miR-137-3p mimic or negative control (mimic NC), were exposed to the STAT3 inhibitor Stattic. A: Transwell assays were conducted to evaluate migratory ability. Scale bar = 100 μm, n = 3; B: Wound healing assays were utilized to assess the migratory response of human umbilical cord mesenchymal stem cell. Scale bar = 200 μm, n = 3; C: The protein levels of CD9, CK19, vimentin, and CD13. The results are illustrated as mean ± SD. ^aP < 0.0001 vs negative control mimic group, ^bP < 0.0001 vs negative control mimic group, ^cP < 0.0001 vs miR-137-3p mimic group, ^dP < 0.0001 vs negative control mimic + Stattic group, n = 3. HucMSC: Human umbilical cord mesenchymal stem cell; NC: Negative control.