Exosomal miR-137-3p targets UBE3C to activate STAT3, promoting migration and differentiation into endometrial epithelial cell of human umbilical cord mesenchymal stem cells under hypoxia

Figure 2 miR-137-3p augmented the migratory and differentiation capabilities of human umbilical cord mesenchymal stem cell into endometrial epithelial cell. Human umbilical cord mesenchymal stem cells were subjected to transfection with miR-137-3p mimic or negative control. A: Transwell assays were employed to assess cellular migration. Scale bar = 100 μm, n = 3; B: Wound healing assays quantified the migratory capacity of human umbilical cord mesenchymal stem cell. Scale bar = 200 μm, n = 3; C: The protein levels of CD9, CK19, vimentin, and CD13 were elucidated using western blotting; D: Western blot analysis elucidating the protein levels of CD9, CK19, vimentin, and CD13. The results are depicted as mean ± SD. ^aP < 0.0001 vs negative control mimic group, n = 3. HucMSC: Human umbilical cord mesenchymal stem cell; NC: Negative control.