DNA methyltransferase 1/miR-342-3p/Forkhead box M1 signaling axis promotes self-renewal in cervical cancer stem-like cells in vitro and nude mice models

Figure 8 Comparing self-renewal related stemness between SiHa or CaSki cells and the corresponding cervical cancer stem cell-like cells. A: DNA methyltransferase 1 (DNMT1) mRNA level in SiHa and CaSki cells and their corresponding cervical cancer stem cell-like cells (CCSLCs); B: MicroRNA (miR)-342-3p level in SiHa and CaSki cells and their corresponding CCSLCs; C: Forkhead box M1 (FOXM1) protein expression level SiHa and CaSki cells and their corresponding CCSLCs with α-tubulin as a loading control; D and E: Representative images of spheres and colonies (left) (scale bars = 100 μm); sphere formation efficiency and colony formation efficiency (right) in SiHa and CaSki cells and their corresponding CCSLCs; F: CD133 and CD49f protein amount with α-tubulin as a loading control; G: SRY-box transcription factor 2 (SOX2) and octamer-binding transcription factor 4 (OCT4) mRNA levels in SiHa and CaSki cells and their corresponding CCSLCs. ^aP < 0.05 vs SiHa cells; ^bP < 0.05 vs CaSki cells (n = 3). SC: Stem cell.