Fat mass and obesity-associated protein in mesenchymal stem cells inhibits osteoclastogenesis via lnc NORAD/miR-4284 axis in ankylosing spondylitis

Figure 6 Targeting fat mass and obesity-associated alleviates enthesitis-related new bone formation in ankylosing spondylitis animal models. A: Histological analysis of bone formation in the enthesis was performed using hematoxylin and eosin (HE) staining and Safranin O. Scale bar = 500 μm (upper) or 250 μm (lower) (n = 5); B: The proposed model shows upregulation of fat mass and obesity-associated in mesenchymal stem cells leads to defects in osteoclastogenesis in ankylosing spondylitis via the long non-coding RNA activated by DNA damage/miR-4284 axis. HE: Hematoxylin and eosin; FTO: Fat mass and obesity-associated; Av-NC: Adeno-associated virus of negative control; Av-shFTO: Adeno-associated virus of short hairpin RNA targeting fat mass and obesity-associated; NORAD: Non-coding RNA activated by DNA damage.