Fat mass and obesity-associated protein in mesenchymal stem cells inhibits osteoclastogenesis via lnc NORAD/miR-4284 axis in ankylosing spondylitis

Figure 5 Fat mass and obesity-associated in mesenchymal stem cells inhibits osteoclastogenesis through the long non-coding RNA activated by DNA damage/miR-4284 axis. A: Representative images of tartrate resistant acid phosphatase (TRAP) staining (× 100) and F-actin assays (× 200); B: Quantitative data of TRAP⁺ osteoclasts number in each well is shown; C: Western blot analysis was performed to detect protein levels of nuclear factor of activated T cells 1 (NFATc1), TRAP, and cathepsin K (CTSK); D: Quantitative data for NFATc1, TRAP, and CTSK protein levels determined by western blot analyses are shown; E: Representative images of TRAP staining (× 100) and F-actin assays (× 200); F: Quantitative data of TRAP⁺ osteoclasts number in each well is shown; G: Western blot analysis was performed to detect protein levels of NFATc1, TRAP, and CTSK; H: Quantitative data for NFATc1, TRAP, and CTSK protein levels determined by western blot analyses are shown. The statistical data are presented as the means ± SDs; n = 3 in each group. ^aP < 0.05, ^bP < 0.01, ^cP < 0.005, ^dP < 0.001. si-NC: Small interfering RNA of negative control; si-FTO: Small interfering RNA targeting fat mass and obesity-associated; OE-NC: Overexpression of negative control; OE-FTO: Overexpression of fat mass and obesity-associated; TRAP: Tartrate resistant acid phosphatase; NFATc1: Nuclear factor of activated T cells 1; CTSK: Cathepsin K; NORAD: Non-coding RNA activated by DNA damage.