Fat mass and obesity-associated protein in mesenchymal stem cells inhibits osteoclastogenesis via lnc NORAD/miR-4284 axis in ankylosing spondylitis

Figure 1 Fat mass and obesity-associated protein expression was upregulated in mesenchymal stem cells from individuals with ankylosing spondylitis compared with mesenchymal stem cells from healthy donors. A: Protein levels of N6-methyladenosine-related methyltransferase and demethylases in mesenchymal stem cells from individuals with ankylosing spondylitis and mesenchymal stem cells from healthy donors were determined by western blot; B: Quantitative data of protein levels of fat mass and obesity-associated, alkB homolog 5, methyltransferase 3, methyltransferase 14, and WT1 associated protein are shown; C: Immunofluorescence staining (scale bar = 100 mm) showed fat mass and obesity-associated expression in the osteocalcin positive mesenchymal stem cells in healthy donors group and ankylosing spondylitis group. The statistical data are represented as the means ± SDs, n = 3 in each group. NS: No significant difference, ^aP < 0.05, ^bP < 0.01. AS-MSCs: Mesenchymal stem cells from individuals with ankylosing spondylitis; HD-MSCs: Mesenchymal stem cells from healthy donors; ALKBH5: AlkB homolog 5; METTL3: Methyltransferase 3; METTL14: Methyltransferase 14; WTAP: WT1 associated protein; OCN: Osteocalcin; FTO: Fat mass and obesity-associated; AS: Ankylosing spondylitis; HD: Healthy donors.