Mesenchymal stem cell exosomes enhance the development of hair follicle to ameliorate androgenetic alopecia

Figure 7 Human adipose-derived mesenchymal stem cell exosomes regulate dermal papillary cell proliferation via cell division cycle protein 42/Wnt/β-catenin pathway in androgenetic alopecia. A: Venn diagram demonstrating the intersections of genes from the androgenetic alopecia gene set and human adipose-derived mesenchymal stem cell exosomes cell cycle gene; B: Five-sino biological pathway enrichment diagram; C: Using single-cell mRNA-sequencing data from the Hair-GEL database, cell types expressing cell division cycle protein 42 (http://hair-gel.net/) were identified; D: Using single-cell mRNA-sequencing data from the Hair-GEL database, cell types expressing glycogen synthase kinase-3β (Gsk3β) (http://hair-gel.net/) were found; E: Representative western blotting image of GSK-3β after human adipose-derived mesenchymal stem cell exosomes was overexpressed in human hair dermal papillary cells; F-H: Statistical analysis of the western blotting in each group. Wnt3a (F), GSK-3β (G) and β-catenin (H). Differences among five groups were assessed by Tukey’s multiple comparison test and one-way ANOVA, and error bars represent SEM. ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001, Compared with control group. hADSC-Exo: Human adipose-derived mesenchymal stem cell exosome; AGA: Androgenetic alopecia; Cdc42: Cell division cycle protein 42; Gsk3b: Glycogen synthase kinase-3β; DHT: Dihydrotestosterone.