Mesenchymal stem cell exosomes enhance the development of hair follicle to ameliorate androgenetic alopecia

Figure 6 Human adipose-derived mesenchymal stem cell exosome improves the hair regeneration in androgenetic alopecia cell model. A: Human hair dermal papillary cells were used in subsequent analyses from passage 3 (P3); B and C: Cell counting kit-8 (CCK8) assay. CCK8 assay was carried out to measure the cell growth in 48 hours (B). Cell survival was determined by the CCK8 assay at 48 hours (C); D and E: The wound healing assay was used to assess migration in cells (D). Statistical analysis of the results of wound healing assay in each group (E); F and G: The migratory properties of human hair dermal papillary cells were analyzed using the Transwell migration assay with Transwell filter chambers (F). Statistical analysis of the results of Transwell migration assay in each group (G). ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. HHDPC: Human hair dermal papillary cell; DHT: Dihydrotestosterone; hADSC-Exo: Human adipose-derived mesenchymal stem cell exosome.