Regulation of lncRNA-ENST on Myc-mediated mitochondrial apoptosis in mesenchymal stem cells: In vitro evidence implicated for acute lung injury therapeutic potential

Figure 3 Long noncoding RNA-ENST affects the apoptosis and autophagy levels of bone marrow mesenchymal stem cells. A: The quantitative polymerase chain reaction analysis revealed the knockdown efficiency of long noncoding RNA (lncRNA)-ENST (n = 3); B: The quantitative polymerase chain reaction analysis showed the overexpression efficiency of lncRNA-ENST (n = 3); C: The Cell Counting Kit-8 assay was used to evaluate cell viability and assess cell damage (n = 3); D: Flow cytometry dual staining was conducted to detect cell apoptosis (apoptosis level statistics chart and flow cytometry chart) (n = 3); E: Western blot analysis was performed to detect the expression of LC3B, ATG7, and ATG5 after knocking down lncRNA-ENST (n = 3). ^aP < 0.001, ^bP < 0.01, ^cP < 0.05. BMSC: Bone marrow mesenchymal stem cell; LncRNA: Long noncoding RNA; NC: Negative control; OE: Overexpression.