Regulation of lncRNA-ENST on Myc-mediated mitochondrial apoptosis in mesenchymal stem cells: In vitro evidence implicated for acute lung injury therapeutic potential

doi:10.4252/wjsc.v17.i3.100079

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Mar 26, 2025 (publication date) through Mar 27, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Mar 26, 2025; 17(3): 100079
Published online Mar 26, 2025. doi: 10.4252/wjsc.v17.i3.100079

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Figure 2 Inverted fluorescence microscopy was used to observe the cell lentiviral transfection efficiency. “Transfection efficiency” was qualitatively evaluated based on green photo fluorescence, with cells exhibiting green photo fluorescence expression considered successfully transfected. “Quantitative analysis” was performed by flow cytometry, where the percentage of green photo fluorescence-positive cells was determined. The transfection efficiency was calculated to be “greater than 80%” based on flow cytometry results. NC: Negative control; PF: Photo fluorescence; GPF: Green photo fluorescence; OE: Overexpression.

Citation: Shen YZ, Yang GP, Ma QM, Wang YS, Wang X. Regulation of lncRNA-ENST on Myc-mediated mitochondrial apoptosis in mesenchymal stem cells: In vitro evidence implicated for acute lung injury therapeutic potential. World J Stem Cells 2025; 17(3): 100079
URL: https://www.wjgnet.com/1948-0210/full/v17/i3/100079.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i3.100079