Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures

Figure 5 Quantification of pathway proteins. A: Measurement of N-cadherin and Wnt1, β-catenin signaling pathway protein expression in each group using western blotting; B: Quantitative analysis of western blotting in each group. ^aP < 0.01 vs two-dimensional-adipose tissue-derived stem cell (N-cadherin), ^bP < 0.001 vs two-dimensional-adipose tissue-derived stem cell-THP1 (N-cadherin), ^cP < 0.001 vs two-dimensional-adipose tissue-derived stem cell (β-catenin), ^dP < 0.001 vs two-dimensional-adipose tissue-derived stem cell-THP1 (β-catenin), ^eP < 0.01 vs two-dimensional-adipose tissue-derived stem cell (Wnt1), ^fP < 0.001 vs two-dimensional-adipose tissue-derived stem cell-THP1 (Wnt1). 3D: Three-dimensional; 2D: Two-dimensional; ADSC: Adipose tissue-derived stem cell.