Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures

doi:10.4252/wjsc.v17.i2.99326

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Feb 26, 2025 (publication date) through Feb 24, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Feb 26, 2025; 17(2): 99326
Published online Feb 26, 2025. doi: 10.4252/wjsc.v17.i2.99326

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Figure 3 Osteogenic differentiation of adipose tissue-derived stem cells. A: Alizarin red staining of adipose tissue-derived stem cell (ADSC) and ADSC-THP1 in two-dimensional culture at 14 and 28 days; B: Semiquantitative measurement of calcium deposits formed during osteogenic differentiation of ADSCs in each group by alizarin red staining colorimetry. ^aP < 0.05 vs day14 two-dimensional-adipose tissue-derived stem cell, ^bP < 0.001 vs day14 three-dimensional-adipose tissue-derived stem cell, ^cP < 0.05 vs day28 two-dimensional-adipose tissue-derived stem cell, ^dP < 0.001 vs day28 three-dimensional-adipose tissue-derived stem cell. 3D: Three-dimensional; 2D: Two-dimensional; ADSC: Adipose tissue-derived stem cell.

Citation: Zhang HA, Zhang BY, Tang HB. Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures. World J Stem Cells 2025; 17(2): 99326
URL: https://www.wjgnet.com/1948-0210/full/v17/i2/99326.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i2.99326