Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures

doi:10.4252/wjsc.v17.i2.99326

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Feb 26, 2025 (publication date) through Feb 24, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Feb 26, 2025; 17(2): 99326
Published online Feb 26, 2025. doi: 10.4252/wjsc.v17.i2.99326

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Figure 1 The isolation, extraction and characterisation of adipose tissue-derived stem cell and THP1. A: Cell culture micrographs of adipose tissue-derived stem cells; B: Cell culture micrographs of THP1; C: Flow cytometry showed adipose tissue-derived stem cells positive for CD29 and CD44, negative for CD31 and CD34; D-F: Osteogenic, adipogenic, and chondrogenic differentiations measured by Alizarin Red staining, Oil Red O staining, and Alcian Blue staining.

Citation: Zhang HA, Zhang BY, Tang HB. Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures. World J Stem Cells 2025; 17(2): 99326
URL: https://www.wjgnet.com/1948-0210/full/v17/i2/99326.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i2.99326