WDR36 inhibits the osteogenic differentiation and migration of periodontal ligament stem cells

Figure 9 Senescence of 0. 1 μg/mL icariin and short hairpin RNA- WD repeat-containing protein 36. A: Senescence-associated β-galactosidase staining results; B: Senescence-associated β-galactosidase staining results showed that the number of positive cells in the icariin (ICA) group was significantly less than that in the group without ICA. By contrast, the number of positive cells in the short hairpin RNA-WD repeat-containing protein 36 (sh-WDR36) group with or without ICA was significantly higher than that in the control group, which indicates that 0.1 μg/mL ICA inhibited the senescence of periodontal ligament stem cells (PDLSCs), and WDR36 depletion promoted the senescence of PDLSCs. The statistical significance was measured by the Student’s t-test. The error bars represent the standard deviation (n = 3). ^bP ≤ 0.01.