WDR36 inhibits the osteogenic differentiation and migration of periodontal ligament stem cells

Figure 7 Osteogenic differentiation of short hairpin RNA-WD repeat-containing protein 36. A: Alkaline phosphatase (ALP) activity results on the third day; B: ALP activity results on the fifth day; C: Alizarin red staining; D: Calcium quantitative analysis; E: Expression of Runt-related transcription factor 2 (RUNX2) in the WD repeat-containing protein 36 (WDR36) depletion group was significantly higher than that in the control group; F: Osterix (OSX) expression in the WDR36 depletion group was significantly higher than that in the control group; G: Distal-less homeobox 3 (DLX3) in the WDR36 depletion group was significantly higher than that in the control group; H: At 0 week and 1 week of osteogenic induction, the expression of dentin matrix protein-1 (DMP1) in the WDR36 depletion group was significantly higher than that in the control group. At 2 weeks, there were no statistically significant differences between the two groups; I: The difference in osteopontin (OPN) expression between the WDR36 depletion group and the control group was not statistically significant. However, after 1 week and 2 weeks of osteogenic induction, OPN expression in the depletion group was significantly higher than that in the control group; J: At 1 week of osteogenic induction, OCN expression in the WDR36 depletion group was significantly higher than that in the control group. However, there were no statistically significant differences between the two groups at 0 week and 2 weeks. The statistical significance was measured by the Student’s t-test. The error bars represent the standard deviation (n = 3). ^aP ≤ 0.05; ^aP ≤ 0.01.