WDR36 inhibits the osteogenic differentiation and migration of periodontal ligament stem cells

Figure 6 Osteogenic differentiation of WD repeat-containing protein 36 overexpression. A: Alkaline phosphatase (ALP) activity results on the third day; B: ALP activity results on the fifth day; C: Alizarin red staining; D: Calcium quantitative analysis; E: Expression of Runt-related transcription factor 2 (RUNX2) in the WD repeat-containing protein 36 (WDR36) overexpression group was significantly lower than that in the control group; F: Osterix (OSX) expression in the WDR36 overexpression group was significantly lower than that in the control group; G: Distal-less homeobox 3 (DLX3) in the WDR36 overexpression group was significantly lower than that in the control group; H: After 1 week and 2 weeks of osteogenic induction, osteopontin (OPN) expression in the WDR36 overexpression group was significantly lower than that in the control group; I: After 1 week of osteogenic induction, the expression of dentin matrix protein-1 (DMP1) in the WDR36 overexpression group was significantly lower than that in the control group. However, there were no statistically significant differences between the two groups at 0 week and 2 weeks; J: After 1 week of osteogenic induction, osteocalcin (OCN) expression in the WDR36 overexpression group was significantly lower than that in the control group. At 0 week and 2 weeks, no statistically significant differences between the two groups were observed. The statistical significance was measured by the Student’s t-test. The error bars represent the standard deviation (n = 3). ^aP ≤ 0.05; ^bP ≤ 0.01.