WDR36 inhibits the osteogenic differentiation and migration of periodontal ligament stem cells

doi:10.4252/wjsc.v17.i2.99132

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Feb 26, 2025 (publication date) through Feb 24, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Feb 26, 2025; 17(2): 99132
Published online Feb 26, 2025. doi: 10.4252/wjsc.v17.i2.99132

Table 1 Real-time polymerase chain reaction primer sequence

Gene	Primer sequences	Primer product size
GAPDH-forward	5’-CATGAGAAGTATGACAACAGCCT-3’	113 bp
GAPDH-reverse	5’-AGTCCTTCCACGATACCAAAGT-3’	113 bp
WDR36-forward	5’-AGCCGTGGATGTTGTTGCTAT-3’	134 bp
WDR36-reverse	5’-GACCATCTGTGCGAAATGAAAT-3’	134 bp
OSX-forward	5’-CTCCTGCGACTGCCCTAAT-3’	126 bp
OSX-reverse	5’-TGCGAAGCCTTGCCATAC-3’	126 bp
RUNX2-forward	5’-CCAACCCACGAATGCACTATC-3’	78 bp
RUNX2-reverse	5’-CGGACATACCGAGGGACATG-3’	78 bp
DLX3-forward	5’-CCAGACGGTGAACCCCTAC-3’	83 bp
DLX3-reverse	5’-CCGACTTGGGCGAGTAAGC-3’	83 bp
OPN-forward	5’-ATGATGGCCGAGGTGATAGTGT-3’	76 bp
OPN-reverse	5’-TACTGGATGTCAGGTCTGCGA-3’	76 bp
OCN-forward	5’-AAGAGACCCAGGCGCTACCT-3’	110 bp
OCN-reverse	5’-AACTCGTCACAGTCCGGATTG-3’	110 bp
DMP1-forward	5’-GAAGAATGGAAGGGTCATTTGG-3’	134 bp
DMP1-reverse	5’-AAGCCACCAGCTAGCCTATAAATGT-3’	134 bp

bp: Base pairs; DLX3: Distal-less homeobox 3; DMP1: Dentin matrix protein-1; OCN: Osteocalcin; OPN: Osteopontin; OSX: Osterix; RUNX2: Runt-related transcription factor 2.

Citation: Wang Y, Zhang FQ, Fan ZP, Zhu XL, Yan WH, Zhang XL. WDR36 inhibits the osteogenic differentiation and migration of periodontal ligament stem cells. World J Stem Cells 2025; 17(2): 99132
URL: https://www.wjgnet.com/1948-0210/full/v17/i2/99132.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i2.99132