Immortalization of epidural fat-derived mesenchymal stem cells: In vitro characterization and adipocyte differentiation potential

Figure 1 Isolation and characterization of epidural fat-derived mesenchymal stem cells. Mesenchymal stem cells were successfully isolated from human epidural fat. A: Graphical representation of the mesenchymal stem cell (MSC) isolation protocol; B: Representative images of epidural fat-derived MSCs (EF-MSCs), scale bars = 200 μm and 50 μm; C: Fluorescence-activated single cell sorting analysis results showing the expression of SC surface markers; D: Representative images and quantification of Oil Red O staining of EF-MSCs, scale bar = 50 μm; E: Expression levels of adipogenesis marker protein CCAAT/enhancer-binding protein alpha. Data are presented as the mean ± standard deviation for each group. ^bP < 0.01.