Bone marrow mesenchymal stem cells promote uterine healing by activating the PI3K/AKT pathway and modulating inflammation in rat models

Table 1 Primer sequences

Gene name	Primer	Sequence	Size
Prss23	Forward	5’-GACTCCTCATCCTTCTCC-3’	208 bp
	Reverse	5’-AGCGGTGTTCCCTTGTG-3’
Table 2	Forward	5’-TACTCAACAGCCCACAGG-3’	100 bp
	Reverse	5’-GATACCCAGCCAGAATGC-3’
Vegfα	Forward	5’-ACACACCCACCCACATAC-3’	243 bp
	Reverse	5’-AGGACGAAAGACCACACC-3’
IL4r	Forward	5’-GTGGAGGAGGAAGAGGA-3’	190 bp
	Reverse	5’-GCAGAAGGGAGGATGAC-3’
Tnfaip8	Forward	5’-CAGGGAAGTGGCTACAG-3’	97 bp
	Reverse	5’-GGTGGCGATGGATTTGG-3’
IL1r1	Forward	5’-CCCACGGAATGAGACGA-3’	247 bp
	Reverse	5’-GCAGATGAACGGATAGCG-3’
IL1α	Forward	5’-GAGTGCTCAGGGAGAAGAC-3’	135 bp
	Reverse	5’-GCTGCGGATGTGAAGTAG-3’
IL1β	Forward	5’-TCTCACAGCAGCATCTCG-3’	184 bp
	Reverse	5’-AGGTCGTCATCATCCCAC-3’
IL4	Forward	5’-CCACGGATGTAACGACAG-3’	205 bp
	Reverse	5’-GTTCTTCAAGCACGGAGG-3’
TNFα	Forward	5’-CGAGATGTGGAACTGGCA-3’	197 bp
	Reverse	5’-GAACTGATGAGAGGGAGC-3’
TGFβ	Forward	5’-CCACTCCCGTGGCTTCTAGT-3’	103 bp
	Reverse	5’-CTTCGATGCGCTTCCGTTTC-3’
FGF	Forward	5’-TTTGCAGCCCTGACCGAGAG-3’	100 bp
	Reverse	5’-GAAGAATCCTCAAGAAGTGGCC-3’
β-actin	Forward	5’-ACCCCGTGCTGCTGACCGAG-3’	250 bp
	Reverse	5’-TCCCGGCCAGCCAGGTCCA-3’

Relative mRNA expression levels were analyzed using the 2^-∆∆Ct method with β-actin as internal references. Prss23: Serine protease 23; Table 2: Transforming growth factor-beta activated kinase 1 (MAP3K7) binding protein 2; Vegfα: Vascular endothelial growth factor alpha; IL4r: Interleukin 4 receptor; Tnfaip8: Tumor necrosis factor alpha induced protein 8; IL1r1: Interleukin 1 receptor type 1; IL1α: Interleukin 1 alpha; IL1β: Interleukin 1 beta; IL4: Interleukin 4; TNFα: Tumor necrosis factor alpha; TGFβ: Transforming growth factor beta; FGF: Fibroblast growth factor.