Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model

Figure 3 Three-dimensional-cultured mesenchymal stromal cells elevate the secretion of prostaglandin E2 and suppress tumor necrosis factor-alpha from lipopolysaccharide-stimulated splenocytes. A: Schematic of the method for collection of supernatant from mesenchymal stromal cells (MSCs) or co-cultured MSCs and lipopolysaccharide-stimulated murine splenocytes; B: Secreted prostaglandin E2 (PGE2) in a MSC culture medium was determined by enzyme linked immunosorbent assay (ELISA) (n = 4 independent experiments, unpaired t-test, ^dP < 0.0001); C: MSCs co-cultured with splenocytes were treated with 10 mmol/L indomethacin (marked as Indo) and secreted PGE2 was assessed using ELISA (n = 3 independent experiment, one-way ANOVA, ^aP < 0.05, ^dP < 0.0001); D: Secreted tumor necrosis factor alpha from MSCs co-cultured with murine splenocytes was assessed by ELISA (n = 3 independent experiment, one-way ANOVA, ^aP < 0.05, ^cP < 0.001, ^dP < 0.0001). 2D: Two-dimensional; 3D: Three-dimensional; PGE2: Prostaglandin E2; LPS: Lipopolysaccharide; TNFα: Tumor necrosis factor alpha.