Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model

Figure 1 Functional polymer-based three-dimensional culture enhances stemness. A: Difference between distribution of mesenchymal stromal cells in the two-dimensional (2D) and 3D culture systems; B: Cell morphology of mesenchymal stromal cells cultured in 2D and 3D. Scale bars represent 50 μm; C: Flow cytometry analysis of stemness markers octamer-binding transcription factor 4 and Nanog homeobox in 2D and 3D. Representative histogram with geometric mean fluorescence intensity were shown. 2D: Two-dimensional; 3D: Three-dimensional; Oct4: Octamer-binding transcription factor 4; Nanog: Nanog homeobox.