Umbilical cord mesenchymal stem cell exosomes alleviate necrotizing enterocolitis in neonatal mice by regulating intestinal epithelial cells autophagy

Figure 3 Human umbilical cord mesenchymal stem cell exosomes attenuate inflammation and injury induced by lipopolysaccharide and enhance the migration ability of intestinal epithelial cells by regulating autophagy. A: The expression of tight junction proteins (ZO-1, claudin-1 and E-cadherin) and cell proliferation proteins in each cell group was determined by western blotting; B: Migration ability of IEC-18 was detected by wound healing assay; C: Inflammatory factor levels in cell supernatants were detected by enzyme-linked immunosorbent assay. Data was expressed as mean ± SEM of three or six independent experiments. ^aP < 0.05 vs control group, ^bP < 0.01 vs control group, ^cP < 0.001 vs control group; ^dP < 0.05 vs lipopolysaccharide, ^eP < 0.01 vs lipopolysaccharide, ^fP < 0.001 vs lipopolysaccharide; ^gP < 0.01 vs Rap. LPS: Lipopolysaccharide; Ctr: Control; IL: Interleukin; TNF: Tumor necrosis factor.